Thermo Fisher Scientific FLI1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunoprecipitation (IP)

2 µg/mL

-

ChIP assay (ChIP)

Assay-dependent

-

ChIP-sequencing (ChIP-Seq)

Assay-dependent

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from C-terminus of human FLI-1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2106100

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is angiosarcoma, Ewing`s sarcoma or placenta tissue.

Target Information

ETS-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes ETS-1, ETS-2, ERG-1, ERG-2, ELK, E74, FLI-1, PU. 1 and PEA3. Members of the ETS gene family exhibit varied pattern of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.