Thermo Fisher Scientific FLI1 Polyclonal Antibody
FLI1 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, WB, IHC, IP, ChIP 등 다양한 응용에 적합합니다. Rabbit IgG 기반으로 인간, 마우스, 랫트 반응 예측. 액상 형태로 -20°C 보관하며, 연구용으로만 사용됩니다.
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Thermo Fisher Scientific FLI1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunoprecipitation (IP) | 2 µg/mL |
| ChIP assay (ChIP) | Assay-dependent |
| ChIP-sequencing (ChIP-Seq) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of human FLI-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2106100 |
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
Suggested positive controls include angiosarcoma, Ewing’s sarcoma, or placenta tissue.
Target Information
ETS-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus.
This family includes ETS-1, ETS-2, ERG-1, ERG-2, ELK, E74, FLI-1, PU.1, and PEA3.
Members of the ETS gene family exhibit varied patterns of tissue expression and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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