Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (1B7), Biotin, eBioscience

Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (1B7), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• eFluor 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1) is a 40 kDa cell-surface adhesion molecule involved in homophilic, calcium-independent cell-cell interactions. It is a type-I transmembrane protein expressed mainly on the basolateral surface of most epithelia.

Normal epithelia express low levels of EpCAM, but increased expression correlates with enhanced proliferation and transition to a mesenchymal phenotype. EpCAM serves as a diagnostic marker for circulating metastatic carcinoma cells, while non-epithelial cancer cells do not express EpCAM.

Applications Reported:
This 1B7 antibody is reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections.

Applications Tested:

• Flow cytometric analysis of A549 cell line: ≤ 0.25 µg/test (100 µL final volume; 10⁵–10⁸ cells/test).
• Immunohistochemistry of frozen tissue: ≤ 10 µg/mL.
  Antibody should be titrated for optimal performance.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It mediates Ca²⁺-independent homotypic interactions and influences cell cycle, proliferation, and metabolism through induction of proto-oncogene c-myc and cyclin A/E.

Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression is associated with enhanced epithelial proliferation and is a marker for epithelial tumors. Ep-CAM is expressed on basolateral surfaces in most simple epithelia and carcinoma types, and distinguishes adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.