Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (1B7), Biotin, eBioscience
인간 EpCAM(CD326)을 인식하는 mouse monoclonal antibody(1B7)로 Biotin 결합형 제품입니다. Flow cytometry 및 IHC(Frozen) 등 다양한 응용에 적합하며, 0.5 mg/mL 농도로 제공됩니다. EpCAM 발현 연구 및 암세포 표지용으로 활용됩니다.
- 카탈로그번호
- 13-9326-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (1B7), Biotin, eBioscience
Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (1B7), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 19 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1B7 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2016625 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- eFluor 660
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1) is a 40 kDa cell-surface adhesion molecule involved in homophilic, calcium-independent cell-cell interactions. It is a type-I transmembrane protein expressed mainly on the basolateral surface of most epithelia.
Normal epithelia express low levels of EpCAM, but increased expression correlates with enhanced proliferation and transition to a mesenchymal phenotype. EpCAM serves as a diagnostic marker for circulating metastatic carcinoma cells, while non-epithelial cancer cells do not express EpCAM.
Applications Reported:
This 1B7 antibody is reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections.
Applications Tested:
- Flow cytometric analysis of A549 cell line: ≤ 0.25 µg/test (100 µL final volume; 10⁵–10⁸ cells/test).
- Immunohistochemistry of frozen tissue: ≤ 10 µg/mL.
Antibody should be titrated for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It mediates Ca²⁺-independent homotypic interactions and influences cell cycle, proliferation, and metabolism through induction of proto-oncogene c-myc and cyclin A/E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression is associated with enhanced epithelial proliferation and is a marker for epithelial tumors. Ep-CAM is expressed on basolateral surfaces in most simple epithelia and carcinoma types, and distinguishes adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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