Thermo Fisher Scientific AIRE Monoclonal Antibody (TM-724), Biotin, eBioscience
Human AIRE 단백질을 인식하는 TM-724 모노클론 항체로, Biotin 결합형 제품입니다. 면역조직화학(IHC 및 IHC-P)에 적합하며, FFPE 조직에서 20 µg/mL 이하 농도로 테스트되었습니다. Rat IgG2a, kappa 아이소타입이며, 4°C 암소 보관 권장.
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Thermo Fisher Scientific AIRE Monoclonal Antibody (TM-724), Biotin, eBioscience
Applications and Tested Dilution
- Immunohistochemistry (IHC)
Publications available: View publication - Immunohistochemistry (Paraffin) (IHC-P)
Tested dilution: 20 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TM-724 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572813 |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
This TM-724 monoclonal antibody reacts with human Autoimmune Regulator (AIRE), a 58 kDa transcription factor highly expressed within the perinuclear region of mature medullary thymic epithelial cells (mTECs).
AIRE mRNA has been observed in lymph node, fetal liver, and intestines, as well as in dendritic cells, epithelial cells, and PBMCs.
AIRE is involved in thymocyte development and regulation of tissue-specific antigen expression during negative selection, as well as chemokine expression and antigen presentation.
Three isoforms (AIRE-1, AIRE-2, AIRE-3) have been reported, all predicted to be recognized by TM-724.
Mutations in AIRE are associated with autoimmune-polyendocrinopathy-candidiasis-ectodermal-dystrophy (APECED) and autoimmune polyglandular syndrome type 1 (APS-1).
Applications Reported:
- Immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections
- Immunohistochemical staining
Applications Tested:
- Tested by IHC on FFPE human thymus at ≤20 µg/mL
- Low pH antigen retrieval is optimal; high pH antigen retrieval also usable
- Recommended to titrate for optimal performance
Target Information
AIRE is a transcriptional regulator protein that binds to DNA as a dimer and tetramer, not as a monomer, and regulates gene expression.
It is a nuclear 545 amino acid protein with HSR domain, two PHD-type zinc fingers, and a SAND domain, forming high-molecular-weight complexes.
AIRE interacts with transcriptional coactivators CBP and CREBBP, forms homodimers/tetramers, and nuclear bodies.
Three isoforms are reported, one predicted to undergo nuclear-mediated decay (NMD).
AIRE mediates E3 ubiquitin ligase activity and defects cause the rare autosomal-recessive autoimmune disease APECED.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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