Thermo Fisher Scientific NR4A1 Monoclonal Antibody (H1648)
NR4A1 단백질을 특이적으로 인식하는 Mouse Monoclonal Antibody (Clone H1648). Western blot, ELISA, Immunoprecipitation에 사용 가능. Baculovirus 발현 인간 NGFI-Bα 항원을 이용해 제작. 비결합형 액상 형태로 2-8°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 0.3 µg/mL |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | H1648 |
| Immunogen | Baculovirus-expressed recombinant human NGFI-Bα (aa 2–249) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 1 month. For long-term storage, store at –20°C. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532202 |
Product Specific Information
This antibody specifically recognizes human NGFI-B-alpha but does not recognize human NGFI-B-beta or gamma.
Target Information
NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. NGFI-B-alpha is expressed in brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung, and prostate. Its expression is induced in response to various stress stimuli and growth factors.
The encoded protein acts as a nuclear transcription factor involved in mediating apoptotic signaling in thymocytes and tumor cells, and plays a role in signaling in the hypothalamic-pituitary axis. Translocation of the protein from the nucleus to mitochondria induces apoptosis.
Three transcript variants encoding two distinct isoforms have been identified for NR4A1. Variant (3), also known as TRC beta, contains multiple differences compared to variant 1. The resulting isoform b contains a shorter and distinct C-terminus compared to isoform a. RXR has been shown to be a partner for NR4A1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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