Takara Cellartis enhanced hiPS-HEP iPSC-derived hepatocytes

Cellartis enhanced hiPS-HEP v2 kits provide an inexhaustible supply of mature hepatocytes derived from human induced pluripotent stem (hiPS) cells. hiPS-HEP cells have functional drug-metabolizing machinery over a 14-day assay window, making them ideal for in vitro drug discovery, drug metabolism, and toxicology-related studies. Mature, robust metabolic features support metabolic disease modeling.

Cellartis enhanced hiPS-HEP v2 kits provide an inexhaustible supply of mature hepatocytes derived from human induced pluripotent stem (hiPS) cells. hiPS-HEP cells have functional drug-metabolizing machinery over a 14-day assay window, making them ideal for in vitro drug discovery, drug metabolism, and toxicology-related studies. Mature, robust metabolic features support metabolic disease modeling.

The user-friendly kit format includes complete media and components for long-term assays. Compared to other hepatic cell models, these cells survive longer in culture, which enables a longer assay window in chronic toxicity experiments and for metabolic disease modeling.

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Overview

• Complete kit for thawing, plating, and culturing the provided cells
• Highly homogeneous population with consistent performance between batches and over time
• Cells are functional and stable for a 14-day assay window
• Cells show long-term, stable CYP450 activities
• Cells express key hepatocyte proteins, including α1AT, Alb, CK18, HNF4α, and E-cadherin
• Cells secrete physiologically relevant levels of albumin and urea
• Cells demonstrate functional insulin and glucose regulation
• Cells show Phase I and II enzyme activities
• Cells demonstrate LDL uptake

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Applications

• Toxicity testing, including chronic toxicity assays
• Drug discovery and development
• Metabolic studies
• Metabolic disease modeling

Components

• 1 vial of ~1.2 x 107 cells
• Hepatocyte thawing medium
• Hepatocyte coating
• Hepatocyte plating medium
• Hepatocyte washing medium
• Hepatocyte long-term maintenance medium

Additional information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.