Thermo Fisher Scientific LGR5 Monoclonal Antibody (UMAB211), UltraMAB
LGR5 단백질을 인식하는 UMAB211 클론의 단일클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. 인간 시료 반응성, 마우스 IgG2a 아이소타입. 고순도의 친화 크로마토그래피 정제. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
| ChIP assay (ChIP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB211 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 22–543 of human LGR5 produced in HEK293 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
G protein-coupled receptor GPR49 (LGR5) is expressed in brain, skeletal muscle, placenta, and spinal cord. ESTs have been isolated from normal human brain (amygdala), embryo, and placenta libraries, as well as from germ cell, uterus, and brain libraries.
G-protein Coupled Receptors (GPCRs) are one of the largest families of signaling molecules, with more than a thousand members predicted. They share a structural motif of seven membrane-spanning helices and exist in both active and inactive forms.
Various ligands activate GPCRs, leading to signaling via G-proteins and downstream effectors. Studies also describe mechanisms of GPCR negative regulation, which are important for cellular physiology and pharmacological research.
Notably, GPCR-targeted drugs such as Zyprexa (Eli Lilly) and Claritin (Schering-Plough) have achieved significant success in mental health and allergy therapeutics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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