Thermo Fisher Scientific VEGF Receptor 2 Polyclonal Antibody
VEGFR-2 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot 및 Immunocytochemistry에 적합하며, 높은 특이성과 재현성을 제공합니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide (coupled to carrier protein) corresponding to amino acids from the N-terminal region of human VEGFR-2. This sequence has high homology to rat and mouse VEGFR-2, and no significant homology to other VEGFR family members. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2943018 |
Product Specific Information
The antibody detects a 180 kDa band corresponding to VEGFR-2 in western blots of human endothelial cells, and shows strong reactivity toward recombinant human VEGFR-2, but not VEGFR-1 or VEGFR-3.
It is also suitable for immunofluorescent staining of VEGFR-2 in HUVEC cells.
Target Information
KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase that binds vascular endothelial growth factor (VEGF) with high affinity. It is expressed early in development by endothelial cell precursors.
FLK1 (VEGF Receptor 2) encodes one of the two receptors of VEGF and is known as kinase insert domain receptor.
The signaling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin αVβ3, and T-cell protein tyrosine phosphatase.
Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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