Thermo Fisher Scientific Phospho-N-WASP (Ser484, Ser485) Polyclonal Antibody
Phospho-N-WASP (Ser484/Ser485) 인산화 단백질을 검출하는 Rabbit Polyclonal 항체. Western blot 및 Immunocytochemistry에 적합. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-N-WASP (S484/S485) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 484 and 485 of human N-WASP. The human WASP sequence has a similar peptide sequence surrounding serine 483 and 484. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2943021 |
Product Specific Information
This antibody was cross-adsorbed to unphosphorylated-N-WASP (S484/S485) peptide and then affinity purified using phospho-N-WASP (S484/S485) peptide.
It detects a 63 kDa protein corresponding to phosphorylated WASP on SDS-PAGE immunoblots of Jurkat cell lysate.
In rat brain, A431, human endothelial, and SKN-SH cells, it detects a 65 kDa protein corresponding to phosphorylated N-WASP.
Target Information
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structures and are involved in transducing signals from cell surface receptors to the actin cytoskeleton.
These proteins contain multiple regulatory motifs and interact with various signaling molecules.
They associate with the small GTPase Cdc42, which regulates actin filament formation and the cytoskeletal organizing complex Arp2/3.
The WASL gene product, a homolog of WAS protein, is ubiquitously expressed with highest levels in neural tissues.
It binds directly to Cdc42 and induces the formation of long actin microspikes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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