Thermo Fisher Scientific Histone H1.5 Polyclonal Antibody
Human Histone H1.5 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF, ChIP 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제되었으며 PBS/BSA buffer에 보관. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:800 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| ChIP assay (ChIP) | 2 µg per 25 µg of chromatin |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide conjugated to KLH derived from within residues 1–100 of Human Histone H1.5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10986457 |
Product Specific Information
Heat mediated antigen retrieval recommended prior to tissue staining.
Target Information
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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