Thermo Fisher Scientific Histone H1.5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:800

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

ChIP assay (ChIP)

Use 2 µg/25 µg of chromatin

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H1.5. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Histone H1.5,uniProtId:P16401-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:226,antigenImageFileName:PA5-19487_Histone_H1.5_P16401-1_Rabbit.svg,antigenImageFileNamePDP:PA5-19487_Histone_H1.5_P16401-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10986457

Product Specific Information

Heat mediated antigen retrieval recommended prior to tissue staining.

Target Information

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.