Thermo Fisher Scientific PARP1 Recombinant Rabbit Monoclonal Antibody (BLR182J)
PARP1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 적합합니다. 인간 및 생쥐 시료에 반응하며, 고순도 정제된 액상 형태로 제공됩니다. 세포 사멸 연구 및 PARP 관련 신호전달 분석에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1 µL/10^6 cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR182J |
| Immunogen | Peptide representing a region between residue 750 and 800 of Human Poly [ADP-ribose] polymerase 1 (PARP1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 50 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunocytochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Target Information
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyze the transfer of ADP-ribose to a variety of nuclear protein acceptors.
Proteolysis of PARP to its stable 85 kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein.
Cleavage occurs between Asp216 and Gly217, a site in PARP conserved across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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