Thermo Fisher Scientific PARP1 Recombinant Rabbit Monoclonal Antibody (BLR183J)
PARP1 단백질을 표적하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, IP에 적합합니다. 고순도 정제 및 안정적 액상 형태로 제공되며, 인간 시료에 반응합니다. 세포 사멸 연구 및 PARP1 관련 신호전달 분석에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Immunoprecipitation (IP) | 6 µL/mg of lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR183J |
| Immunogen | Peptide representing the cleaved N-terminal of Human Poly [ADP-ribose] polymerase 1 (PARP1), region between residue 215–265 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunocytochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Target Information
Poly ADP-Ribose Polymerase (PARP) uses NAD⁺ as a substrate to catalyze the transfer of ADP-ribose to various nuclear protein acceptors. Proteolysis of PARP to its stable 85 kDa fragment is an early marker of apoptosis, mediated by the caspase CPP32 protein. Cleavage occurs between Asp216 and Gly217, a conserved site across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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