Thermo Fisher Scientific AP2M1 Monoclonal Antibody (OTI1B11), TrueMAB
AP2M1 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, ICC/IF, Flow cytometry에 사용 가능. Human 반응성. Lyophilized 형태로 제공되며, PBS buffer(8% trehalose) 포함. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B11 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 97–383 of human AP2M1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882) before use.
Target Information
The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunit family.
The mu 1 subunit (AP2M1) is essential for vacuolar ATPase activity responsible for endosomal and lysosomal acidification.
AP2M1 associates with the HIV-1 protein Nef, suggesting a role in enhancing endocytosis of CD4 and MHC class I molecules.
It may also regulate intracellular trafficking and function of CTLA-4.
At least two isoforms of AP2M1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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