Thermo Fisher Scientific AP2M1 Monoclonal Antibody (OTI1E9), TrueMAB
AP2M1 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, WB, IHC, ICC 등 다양한 응용에 적합합니다. 사람, 생쥐, 개, 랫드 반응성이 있으며, 동결건조 형태로 제공됩니다. 연구용으로만 사용되며, 고순도 친화 크로마토그래피 정제 제품입니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 97–383 of human AP2M1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The heterotetrameric coat assembly protein complex, known as adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family.
The mu 1 subunit (AP2M1) is essential for vacuolar ATPase activity, which drives proton pumping for endosome and lysosome acidification.
AP2M1 associates with HIV-1 protein Nef, potentially enhancing endocytosis of CD4 and MHC class I molecules.
It may also regulate intracellular trafficking and function of CTLA-4 protein.
At least two isoforms of AP2M1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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