Thermo Fisher Scientific DDX4 Monoclonal Antibody (2F9H5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

1:200-1:400

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Protozoa

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2F9H5

Immunogen

Purified recombinant fragment of human DDX4 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11001446

Product Specific Information

MA5-15565 targets DDX4 in FACS, IF, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15565 immunogen is purified recombinant fragment of human DDX4 expressed in E. Coli.

MA5-15565 detects DDX4 which has a predicted molecular weight of approximately 76kDa.

Target Information

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.