Thermo Fisher Scientific EGF Monoclonal Antibody (9D7F11)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA5-15571 targets EGF in indirect ELISA, IHC, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human EGF expressed in E. coli.
MA5-15571 detects EGF, which has a predicted molecular weight of approximately 134 kDa.

Target Information

Epidermal Growth Factor (EGF) binds to the EGF receptor (EGFR), a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to environmental stimuli. Upon EGF binding, EGFR forms homo- or heterodimers with other family members, each activating distinct signaling cascades via SH2-containing effector proteins.
EGF is widely expressed in kidney, brain, prostate, and salivary glands, acting as a potent mitogenic factor. The phosphorylated receptor recruits adapter proteins like GRB2, triggering downstream signaling cascades such as RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT pathways.
EGF may also activate NF-κB signaling. Defects in the EGF gene are linked to hypomagnesemia type 4, and dysregulation has been associated with cancer growth and progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.