Thermo Fisher Scientific POLR2A Monoclonal Antibody (OTI1E5), TrueMAB

Applications and Tested Dilutions

Product Specifications

Target Information

DNA-directed RNA polymerase II subunit RPB1 (POLR2A) is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR2A is the largest subunit and a catalytic component of RNA polymerase II, which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with the second largest subunit.

Pol II is the central component of the basal RNA polymerase II transcription machinery and is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft and clamp element that moves to open and close the cleft, as well as jaws that grab the incoming DNA template. During transcription initiation, the promoter DNA strand is positioned within the central active site cleft of Pol II. A bridging helix from RPB1 crosses the cleft near the catalytic site and promotes translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site.

Transcription elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which serves as a platform for assembly of factors regulating transcription initiation, elongation, termination, and mRNA processing. Gene expression regulation depends on the balance between methylation and acetylation levels of CTD-lysines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.