Thermo Fisher Scientific p38 MAPK alpha Monoclonal Antibody (9F12), FITC
FITC로 표지된 Thermo Fisher Scientific의 p38 MAPK alpha 단클론 항체(9F12). 인간, 마우스, 랫트 반응성. Western blot, IHC, ELISA 등 다양한 응용 가능. 38kDa p38α 단백질 검출에 적합하며 Protein G로 정제된 액상 형태.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:10,000 | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent | |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9F12 |
| Immunogen | Full length recombinant protein expressed in E. coli cells |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931647 |
Additional Formats
Product Specific Information
Detects approximately 38 kDa protein corresponding to p38 alpha MAPK when loaded with 6 ng of purified p38 alpha by chemiluminescent immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 38 kDa.
Target Information
MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates various cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in its nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motif.
There are four isoforms of p38 MAPK, which differ in tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate Thr180 and Tyr182 residues in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2, and other transcription factors through phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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