Thermo Fisher Scientific p38 MAPK alpha Monoclonal Antibody (9F12), APC
p38 MAPK alpha 단백질 검출용 APC 결합 단일클론 항체로, WB, IHC, ELISA 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 단백질 G 정제, 보존제 무첨가. 연구용으로만 사용 가능.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific p38 MAPK alpha Monoclonal Antibody (9F12), APC
Thermo Fisher Scientific p38 MAPK alpha Monoclonal Antibody (9F12), APC
Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent | |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9F12 |
| Immunogen | Full length recombinant protein expressed in E. coli cells |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931646 |
Available Formats
- FITC conjugate (Cat. MA5-45193)
- PerCP conjugate (Cat. MA5-45194)
- Custom conjugation available upon request
Product Specific Information
Detects approximately 38 kDa protein corresponding to p38 alpha MAPK when loaded with 6 ng of purified p38 alpha by chemiluminescent immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 38 kDa.
Target Information
MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates various cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motif. There are four isoforms of p38 MAPK, differing in tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylates Thr180 and Tyr182 residues in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2, and other transcription factors through phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.