Thermo Fisher Scientific HSC70 Monoclonal Antibody (N27F3-4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

1-10 µg/mL

-

Flow Cytometry (Flow)

10 µg/mL

-

Immunoprecipitation (IP)

1-10 µg/mL

-

Product Specifications

Species Reactivity

Human, Many, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N27F3-4

Immunogen

Purified recombinant Hsp70/Hsc70.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_906423

Product Specific Information

MA1-10890 detects Heat Shock Protein 70/Heat Shock Cognate Protein 70 in human, mouse, rat, rabbit, monkey, guinea pig, hamster, chicken, bovine, canine, ovine, porcine, fish, Xenopus, Drosophila, C. elegans, and plant samples.

MA1-10890 has been successfully used in Western blot, immunohistochemistry, immunoprecipitation and flow cytometry procedures. By Western blot, MA1-10890 is specific for the ~72 kDa Heat Shock Protein 70/Heat Shock Cognate Protein 70 protein. Positive control of HeLa cell lysate recommended.

MA1-10890 immunogen is purified recombinant Hsp70/Hsc70.

Target Information

The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73) is expressed constitutively and is slightly heat-inducible. HSC70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. HSC70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.