Thermo Fisher Scientific IPO5 Monoclonal Antibody (N9C1)

Applications

Western Blot (WB)

• Tested Dilution: 1–10 µg/mL
• View 1 publication

Product Specifications

Product Specific Information

• MA1-10886 detects PSE1/Ku Protein from human samples.
• Validated for use in Western blot applications.
• Immunogen: Proximal Sequence Element (PSE1) of the human U1 small nuclear RNA extracted from human placenta.

Target Information

Nucleocytoplasmic transport is a signal- and energy-dependent process occurring through nuclear pore complexes embedded in the nuclear envelope.
Import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits (karyopherins).

• Importin alpha binds NLS-containing cargo in the cytoplasm.
• Importin beta docks the complex at the cytoplasmic side of the nuclear pore complex.
• In the presence of nucleoside triphosphates and Ran (a small GTP-binding protein), the complex translocates into the nucleus and dissociates.
• Importin alpha enters the nucleoplasm with its passenger protein, while importin beta remains at the pore.
• Interactions between importin beta and FG repeats of nucleoporins are essential for translocation through the pore complex.

The encoded protein is a member of the importin beta family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.