Thermo Fisher Scientific RWDD3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Immunogen sequence: ESLLSEPMVH ELVLWIQQNL RHILSQPETG SGSEKCTFST STTMDDGLWI (aa 92-141). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RWDD3,uniProtId:Q9Y3V2-1,ncbiNodeId:9606,antigenRange:92-141,antigenLength:267,antigenImageFileName:PA5-114147_RWDD3_Q9Y3V2-1_Rabbit.svg,antigenImageFileNamePDP:PA5-114147_RWDD3_Q9Y3V2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2884662

Product Specific Information

Immunogen sequence: ESLLSEPMVH ELVLWIQQNL RHILSQPETG SGSEKCTFST STTMDDGLWI

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Predicted homology: Dog: 86%; Guinea Pig: 86%; Human: 100%; Mouse: 79%; Rat: 86%

Target Information

RWDD3 (RSUME), a small RWD-containing protein, has a central role in sumoylation by enhancing SUMO conjugation in the regulatory network of immune-inflammatory signals. RWDD3 increases Ikappa-Beta sumoylation and stability. In addition, RWDD3 inhibits TNF-a-induced kappaB-LUC (Luciferase) reporter activity, showing the functional consequence of Ikappa-Beta increased stability. RSUME-enhanced sumoylation of Ikappa-Beta leads to the inhibition of NF-kappa-Beta activity on two well-known inflammatory genes, IL-8 and cyclooxygenase-2 (Cox-2) and therefore may also favor anti-inflammatory pathways. Expression of RWDD3 was induced under hypoxic conditions and it has a potential role during vascularization. Both BMP-4 and RWDD3 may be interesting targets for inhibiting steps involved in pituitary tumorigenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.