Thermo Fisher Scientific NFkB p52/p100 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 730-900 of NF-κB2 (NP_0010709621) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NFkB p52/p100,uniProtId:Q00653-1,ncbiNodeId:9606,antigenRange:730-900,antigenLength:900,antigenImageFileName:PA5-120927_NFkB_p52p100_Q00653-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120927_NFkB_p52p100_Q00653-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.63 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914499

Product Specific Information

Positive test controls include: HeLa, MCF7, NIH/3T3, C6, Mouse thymus. The target is usually found in the following locations: Cytoplasm, Nucleus.

Immunogen sequence: GHTPLDLTCS TKVKTLLLNA AQNTMEPPLT PPSPAGPGLS LGDTALQNLE QLLDGPEAQG SWAELAERLG LRSLVDTYRQ TTSPSGSLLR SYELAGGDLA GLLEALSDMG LEEGVRLLRG PETRDKLPST AEVKEDSAYG SQSVEQEAEK LGPPPEPPGG LCHGHPQPQV H

Target Information

The NFkB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFkB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFkB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.