Thermo Fisher Scientific Cyclin B1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Keep as concentrated solution.
• Predicted reactivity: Pig (85%), Rhesus Monkey (97%), Bovine (82%).
• Positive Control: U2OS, U2OS treated 100 ng/mL Nocodazole 16 hr, HeLa, T84, CL1-5, CL1-0, A549, HUVEC.
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs). Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis and essential for cell cycle control at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF).
Two alternative transcripts exist: one constitutively expressed and one cell cycle-regulated, expressed predominantly during G2/M phase. Cyclin B1 accumulates during G2 phase and undergoes APC-dependent destruction at the end of mitosis, which is required for cell cycle progression.
Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.

Safety Information

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.