Thermo Fisher Scientific RhoA Polyclonal Antibody
RhoA 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ELISA 등에 사용 가능. Human, Mouse, Rat 반응성. 고순도 Affinity Chromatography 정제, 안정적 PBS/glycerol buffer 제공.
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Thermo Fisher Scientific RhoA Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 94–193 of human RhoA (NP_001655.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.77 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913815 |
Product Specific Information
- Positive test controls: NIH/3T3, Mouse lung
- Target localization: Cell membrane, Cell projection, Cleavage furrow, Cytoplasm, Cytoplasmic side, Lipid-anchor, Midbody, Cell cortex, Cytoskeleton, Lamellipodium
Immunogen sequence:
NIPEKWTPEV KHFCPNVPII LVGNKKDLRN DEHTRRELAK MKQEPVKPEE GRDMANRIGA FGYMECSAKT KDGVREVFEM ATRAALQARR GKKKSGCLVL
Target Information
RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.
It is involved in microtubule-dependent signaling required for myosin contractile ring formation during cytokinesis and plays an essential role in cleavage furrow formation.
RhoA is critical for apical junction formation in keratinocyte adhesion and serves as a target for yopT cysteine peptidase from Yersinia pestis and Yersinia pseudotuberculosis.
It stimulates PKN2 kinase activity and may activate PLCE1.
Activated by ARHGEF2, RhoA promotes GDP/GTP exchange and is essential for SPATA13-mediated regulation of cell migration and adhesion dynamics.
The MEMO1–RHOA–DIAPH1 pathway contributes to ERBB2-dependent microtubule stabilization at the cell cortex, controlling localization of APC and CLASP2 via GSK3B regulation.
Membrane-bound APC enables MACF1 localization, necessary for microtubule capture and stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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