Thermo Fisher Scientific AP2M1 Polyclonal Antibody
Rabbit 폴리클로날 항체로 Human, Mouse, Rat 시료에서 AP2M1 단백질 검출에 사용 가능. Western blot 및 ELISA에 적합하며, 고순도 친화 크로마토그래피 정제. 세포막 및 세포질막 단백질 연구에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 134–433 of human AP2M1 (NP_004059.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.43 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913816 |
Product Specific Information
- Positive test controls: HeLa, Mouse brain, Rat brain
- Subcellular localization: Cell membrane, Cytoplasmic side, Membrane, Peripheral membrane protein, coated pit
Immunogen sequence:
TQQGIKSQHQ TKEEQSQITS QVTGQIGWRR EGIKYRRNEL FLDVLESVNL LMSPQGQVLS AHVSGRVVMK SYLSGMPECK FGMNDKIVIE KQGKGTADET SKSGKQSIAI DDCTFHQCVR LSKFDSERSI SFIPPDGEFE LMRYRTTKDI ILPFRVIPLV REVGRTKLEV KVVIKSNFKP SLLAQKIEVR IPTPLNTSGV QVICMKGKAK YKASENAIVW KIKRMAGMKE SQISAEIELL PTNDKKKWAR PPISMNFEVP FAPSGLKVRY LKVFEPKLNY SDHDVIKWVR YIGRSGIYET
Target Information
The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family.
The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping in endosome and lysosome acidification.
AP2M1 associates with the HIV-1 protein Nef, enhancing endocytosis of CD4 and MHC class I molecules.
It also plays a role in regulating intracellular trafficking and function of CTLA-4 protein.
At least two isoforms of AP2M1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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