Thermo Fisher Scientific AP2M1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 134-433 of human AP2M1 (NP_004059.2). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AP2M1,uniProtId:Q96CW1-1,ncbiNodeId:9606,antigenRange:134-433,antigenLength:435,antigenImageFileName:PA5-120244_AP2M1_Q96CW1-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120244_AP2M1_Q96CW1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2913816

Product Specific Information

Positive test controls include: HeLa, Mouse brain, Rat brain . The target is usually found in the following locations: Cell membrane, Cytoplasmic side, Membrane, Peripheral membrane protein, coated pit.

Immunogen sequence: TQQGIKSQHQ TKEEQSQITS QVTGQIGWRR EGIKYRRNEL FLDVLESVNL LMSPQGQVLS AHVSGRVVMK SYLSGMPECK FGMNDKIVIE KQGKGTADET SKSGKQSIAI DDCTFHQCVR LSKFDSERSI SFIPPDGEFE LMRYRTTKDI ILPFRVIPLV REVGRTKLEV KVVIKSNFKP SLLAQKIEVR IPTPLNTSGV QVICMKGKAK YKASENAIVW KIKRMAGMKE SQISAEIELL PTNDKKKWAR PPISMNFEVP FAPSGLKVRY LKVFEPKLNY SDHDVIKWVR YIGRSGIYET

Target Information

The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.