Thermo Fisher Scientific VCAM-1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

5-15 µg/mL

-

Flow Cytometry (Flow)

0.25 µg/10^6 cells

-

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VCAM-1/CD106 Phe25-Glu698 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:VCAM-1 (CD106),uniProtId:P29533-1,ncbiNodeId:10090,antigenRange:25-698,antigenLength:739,antigenImageFileName:PA5-47029_VCAM-1_CD106_P29533-1_Goat.svg,antigenImageFileNamePDP:PA5-47029_VCAM-1_CD106_P29533-1_Goat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609027

Product Specific Information

In direct ELISA and Western blots, approximately 5% cross-reactivity with recombinant human VCAM-1 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.