Thermo Fisher Scientific CD122 Monoclonal Antibody (TM-b1 (TM-beta1)), APC, eBioscience

Thermo Fisher Scientific CD122 Monoclonal Antibody (TM-b1 (TM-beta1)), APC, eBioscience™

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.25 µg/test
  View 2 publications

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation available

Product Specific Information

The TM-b1 monoclonal antibody reacts with mouse CD122, the 90–110 kDa interleukin-2 receptor beta chain (IL-2R beta). CD122 is expressed by NK cells and some peripheral T cells, and its expression is upregulated upon activation.
CD122 associates with CD25 and CD132 (common gamma chain) to form the high-affinity IL-2 receptor. TM-b1 inhibits high-affinity IL-2 binding to the receptor.

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Tested by flow cytometric analysis of mouse splenocytes
• Recommended ≤0.25 µg per test in 100 µL final volume
• Cell number: 10⁵–10⁸ cells/test (empirical optimization recommended)
• Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD122 (Interleukin 2 receptor beta chain) is involved in T cell-mediated immune responses, primarily expressed in the hematopoietic system and exists in three forms:

• Low affinity: alpha subunit monomer (no signal transduction)
• Intermediate affinity: alpha/beta heterodimer
• High affinity: alpha/beta/gamma heterotrimer

Intermediate and high affinity forms participate in receptor-mediated endocytosis and mitogenic signaling.
CD122 encodes a type I membrane protein.
Associated diseases: oligoarticular juvenile idiopathic arthritis, rheumatoid factor negative juvenile idiopathic arthritis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

제품 이미지