Thermo Fisher Scientific KSHV ORF45 Monoclonal Antibody (2D4A5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2D4A5

Immunogen

Purified recombinant fragment of KSHV ORF45 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10999794

Product Specific Information

MA5-14769 targets KSHV ORF45 in indirect ELISA, IHC and WB applications and shows reactivity with Virus samples.

The MA5-14769 immunogen is purified recombinant fragment of KSHV ORF45 expressed in E. Coli.

Target Information

Kaposis sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposis sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman`s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.