Thermo Fisher Scientific CD14 Monoclonal Antibody (5A3)
인간 CD14 단백질을 인식하는 마우스 단클론 항체로, WB, IHC, Flow Cytometry, ELISA 등 다양한 응용에 사용 가능. 비결합형 액상 형태로 제공되며, 단기 4°C, 장기 -20°C 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5A3 |
| Immunogen | Purified recombinant fragment of human CD14 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10983091 |
Product Specific Information
MA5-14773 targets CD14 in indirect ELISA, FACS, and IHC applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human CD14 expressed in E. coli.
Target Information
CD14 is a 55 kDa GPI-anchored glycoprotein expressed on mature monocytes, macrophages, and neutrophils.
It functions as a multifunctional lipopolysaccharide receptor and exists in both membrane-bound and soluble forms.
CD14 binds lipopolysaccharides in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), aiding in immune recognition and response.
Soluble CD14 (sCD14) helps neutralize serum lipopolysaccharides and can bind apoptotic cells, influencing allergic, inflammatory, and infectious processes.
Alternative splicing produces multiple transcript variants encoding the same CD14 isoform.
CD14 dysfunction is associated with conditions such as Mycobacterium chelonae infection and croup.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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