Thermo Fisher Scientific CD123 Monoclonal Antibody (6H6), Brilliant Ultra Violet 615, eBioscience
인간 CD123 (IL-3 수용체 α사슬)을 인식하는 6H6 단클론 항체로, Brilliant Ultra Violet 615 형광체 결합. 유세포분석용으로 사전 희석되어 테스트 완료. 광산화에 민감하며 자외선(355 nm) 레이저 사용 장비에 적합. 연구용 전용.
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Thermo Fisher Scientific CD123 Monoclonal Antibody (6H6), Brilliant Ultra Violet 615, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.125 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6H6 |
| Conjugate | Brilliant Ultra Violet™ 615 |
| Excitation / Emission Max | 350 / 607 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2929049 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 480
- Brilliant Violet 786
- eFluor 450
- FITC
- NovaFluor Blue 510
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60–70 kDa transmembrane protein binds IL-3 with low affinity by itself, and with CD131 (common beta chain) forms a high-affinity receptor. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells.
Use at 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Fluorochrome Information:
Brilliant Ultra Violet™ 615 (BUV615) emits at 615 nm and is intended for use on cytometers equipped with a UV (355 nm) laser. Ensure instrument compatibility.
Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
Light Sensitivity:
Sensitive to photo-induced oxidation. Protect vial and samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal fluorescence loss.
Excitation: 350 nm
Emission: 615 nm
Laser: Ultraviolet Laser
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
IL-3Ra (Interleukin-3 receptor subunit alpha) is a type I cytokine receptor forming a heterodimer with the common beta (CDw131) subunit. It transmits IL-3 signals and is overexpressed on acute myeloid leukemia (AML) blasts compared to normal hematopoietic cells, representing a potential therapeutic target.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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