Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (2E7), Brilliant Ultra Violet 615, eBioscience

Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (2E7), Brilliant Ultra Violet 615, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 563
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 711
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Request custom conjugation

Product Specific Information

The 2E7 monoclonal antibody reacts with mouse CD103, the alphaE integrin. CD103 non-covalently associates with integrin beta7 and is expressed on intraepithelial lymphocytes, a subset of peripheral lymphocytes, and dendritic epidermal T cells (DEC). E-cadherin binds to CD103 and mediates lymphocyte homing to intestinal epithelium.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse lymph node cells, ≤ 0.5 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Titrate for optimal performance.

Brilliant Ultra Violet™ 615 (BUV615) emits at 615 nm and is used with ultraviolet (355 nm) laser-equipped cytometers. Ensure instrument compatibility.

Staining Panel Recommendation:
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Light Sensitivity:
Protect vial and stained samples from light.

Fixation:
Samples may be stored in:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)
  Store up to 3 days in dark at 4°C.

Fluorophore Characteristics:

• Excitation: 350 nm
• Emission: 615 nm
• Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or affiliates, used under license. Powered by Sirigen™.

Target Information

CD103 (ITGAE, Integrin alpha E) is expressed on mucosa-associated and activated T lymphocytes, comprising a 150 kDa alpha chain and a 120 kDa beta chain. It is part of the integrin family, forming E-cadherin binding complexes with beta7 integrin. CD103 is found in intestinal intraepithelial lymphocytes and may act as an adhesion and activation molecule.
Associated diseases include hairy cell leukemia and splenic marginal zone lymphoma.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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