Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Super Bright 780, eBioscience
인간 CD3 복합체의 epsilon-subunit을 인식하는 OKT3 단클론 항체로, Super Bright 780 형광체 결합 제품입니다. Flow cytometry에 최적화되어 있으며, 높은 형광 안정성과 명확한 T세포 표지 가능. 405 nm 레이저에서 여기되어 780 nm에서 방출.
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Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Super Bright 780, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 11 publications |
| Functional Assay | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 780, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT3 |
| Conjugate | Super Bright™ 780 |
| Excitation / Emission Max | 413 / 780 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2724087 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 615, 661, 737, 805
- Brilliant Violet 421, 605
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Blue 510, 585
- NovaFluor Yellow 590, 660, 730
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702
- Request custom conjugation
Product Specific Information
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. It has potent immunosuppressive properties and has been effective in treating renal, heart, and liver allograft rejection. CD3 is expressed by thymocytes and all mature T cells. Crosslinking of TCR initiates intracellular signaling for activation and proliferation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested with human peripheral blood cells at 5 µL (0.5 µg)/test.
Laser: Violet (405 nm); Emission: 780 nm.
Super Bright 780 is a tandem dye excited at 405 nm and emits at 780 nm. Use a 780/60 bandpass filter. Protect from light due to photo-oxidation sensitivity.
Recommended Buffer: Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Fixation: Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer or 1-step Fix/Lyse Solution.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
The CD3 complex transduces antigen-recognition signals in T cells and regulates TCR expression. CD3 subunits (gamma, delta, epsilon, zeta) are encoded on chromosome 11 and mediate signal transduction through tyrosine-based motifs. CD3 is expressed on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes but not on B or NK cells. Defects in CD3 genes are associated with immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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