Thermo Fisher Scientific CD43 Monoclonal Antibody (eBioR2/60), Biotin, eBioscience

Applications

Tested Dilution

• Flow Cytometry (Flow): 0.5 µg/test
• Miscellaneous PubMed (Misc): -

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Product Specifications

Additional Formats

• Unconjugated
• FITC
• PE
• Request custom conjugation

Product Specific Information

The eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by early B cell subsets in bone marrow, thymocytes, mature T cells, NK cells, myeloid lineage cells, and platelets. CD43 binds to CD54 and plays roles in adhesion and B cell survival.

While S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, R2/60 detects both isoforms. R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes, whereas S7 and 1B11 recognize distinct epitopes expressed differently by splenic B and T cells. R2/60 recognizes an epitope shared by both isoforms of CD43.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤0.5 µg/test.
A test is defined as the amount of antibody that stains a cell sample in a final volume of 100 µL. Cell number can range from 10⁵ to 10⁸ cells/test. Antibody should be titrated for optimal performance.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with a high negative charge, expressed on most hematopoietic cells. It contributes to a repulsive barrier that interferes with cellular adhesion but can also promote leukocyte aggregation. Through interactions with actin-binding proteins ezrin and moesin, CD43 regulates T-cell morphology and cell-cell interactions during lymphocyte traffic.

CD43 signaling enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Its expression induces the tumor suppressor p53 protein, but in cases of p53 and ARF deficiency, CD43 promotes tumor proliferation and viability. CD43 is an important modulator of leukocyte functions. Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.