Thermo Fisher Scientific SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• Biotin
• eFluor 660
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The eBioMC-480 (MC-480) antibody reacts with stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed on early mouse embryos, murine embryonal carcinoma (EC) cells, embryonic stem (ES) cells, and murine/human germ cells (EG). No immunoreactivity is seen with undifferentiated human EC and ES cells. Differentiation of human EC increases SSEA-1 expression, while in mouse cells it decreases. SSEA-1 is also expressed on mature human granulocytes (CD15) and some monocytes, and is associated with cell adhesion, migration, and differentiation.

Applications Reported:
Reported for use in flow cytometry, immunoprecipitation, and immunohistochemistry.

Applications Tested:
Tested by flow cytometric analysis of the F9 cell line. Recommended ≤0.5 µg/test in 100 µL volume (10⁵–10⁸ cells/test). Titrate for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD15 (Lewis X, Le(x); SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of various cell types including granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and tumor cells. Variants include sialyl-Lewis x and sulphated Lewis x.
Cells with high Le(x) expression show calcium-dependent self-aggregation, relevant in embryo compaction and teratocarcinoma cell aggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands. CD15 expression is widely used in the diagnosis of Hodgkin’s disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.