Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Brilliant Violet 650, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤ 0.125 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• APC
• Biotin
• Brilliant UV 615 / 661 / 737 / 805
• Brilliant Violet 421 / 786
• Functional Grade
• NovaFluor Red 700 / 755
• NovaFluor Yellow 690 / 700
• PE / PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 780
• Request custom conjugation available

Product Specific Information

The MIH5 monoclonal antibody reacts with mouse B7-H1 (PD-L1), a 40 kDa member of the B7 family belonging to the immunoglobulin superfamily. B7-H1 is expressed on most leukocytes including T, B, NK, and dendritic cells, and acts as a ligand for PD-1. The MIH5 clone is reported to be a blocking antibody.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤ 0.125 µg/test (100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test). Optimal titration recommended.

Fluorochrome Information:
Brilliant Violet™ 650 (BV650) emits at 649 nm, excited by a 405 nm violet laser. Ensure your cytometer supports this fluorochrome.

Buffer Recommendations:
When using multiple Brilliant Violet™, Brilliant Ultra Violet™, or polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect from light to avoid photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark. BV650 is not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 649 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Programmed death receptor ligand 1 (PD-L1, B7-H1) is a B7 family member that binds PD-1, a receptor that negatively regulates T-cell receptor signaling. PD-L1 engagement reduces TCR-mediated proliferation and cytokine production. PD-L1 expression is abundant in many murine and human cancers and can be upregulated by IFN-γ, suggesting a key role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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