Thermo Fisher Scientific Kir3.4 (KCNJ5) Polyclonal Antibody
Kir3.4 (KCNJ5) 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체. Western blot 및 ICC/IF에 적합하며, 인간·마우스·랫트 반응성 보유. 고순도(>95%) 항원 친화 크로마토그래피 정제. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Kir3.4 (KCNJ5) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 374–419 of human Kir3.4 (KCNJ5) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2553839 |
Product Specific Information
- Detects endogenous Kir3.4 protein at ~48 kDa
- Purity >95% by SDS-PAGE
Target Information
G protein-coupled inwardly rectifying potassium channels (Kir3.1–Kir3.4) are associated with numerous neurotransmitter receptors in the brain. They are abundantly expressed in regions such as the olfactory bulb, hippocampus, neocortex, dentate gyrus, cerebellar cortex, and thalamus.
Also known as GIRK channels, Kir3 proteins localize to soma, dendrites, and axons of neurons. Gβγ subunits released from G protein heterotrimers regulate Kir3 channel activity: Gβ3/Gβ4-containing dimers enhance K⁺ current, while Gβ5-containing dimers inhibit it. Kir3 activity is also inhibited by tyrosine phosphorylation. Brain-derived neurotrophic factor (BDNF) activates receptor tyrosine kinase B, which phosphorylates Kir3 tyrosine residues, inactivating the channel.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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