Thermo Fisher Scientific RNF168 Polyclonal Antibody

Applications

Immunocytochemistry (ICC/IF)

• Tested dilution: 0.25–2 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:

LTPKSQFGSAS HSEAVQEVRK DSVSKDIDSS DRKSPTGQDT 
EIEDMPTLSP QISLGVGEQG ADSSIESPMP WLCACGAEWY 
HEGNVKTRPS NHGK

• Highest antigen sequence identity to the following orthologs:
  • Mouse: 43%
  • Rat: 42%

Target Information

RNF168 is a chromatin-associated RING finger protein functioning as a ubiquitin ligase both in vitro and in vivo. It targets histones H2A and H2AX (not H2B), forming K63-linked polyubiquitin chains. Upon DNA double-strand breaks, RNF168 is recruited to damage sites where it co-localizes with γH2AX and 53BP1 in an RNF8-dependent manner. This process enhances the accumulation of ubiquitinated proteins necessary for the retention of 53BP1 and BRCA1, supporting DNA damage response signaling. RNF168 thus plays a critical role in genome maintenance, with at least three known isoforms.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.