Thermo Fisher Scientific proNGF Polyclonal Antibody
proNGF 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot 및 IHC에 적합하며, PBS 기반 무방부제 동결건조 형태입니다. 장기 보관 시 -20°C에서 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 10–50 µg/mL | - |
| Immunohistochemistry (IHC) | 10–50 µg/mL | - |
| Miscellaneous (PubMed) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from human proNGF conjugated to an immunogenic carrier protein was used as the antigen |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: proNGF.
Target Information
Nerve growth factor (NGF) influences the survival and differentiation of various neuronal and non-neuronal cells. It binds to specific cell surface receptors with high and low affinity. Only the high-affinity receptor mediates signal transduction.
Cross-linking studies with 125I-NGF have shown that the high-affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF, while the low-affinity receptor forms an Mr 100,000 complex.
Full-length cDNA clones for both human and rat NGF receptor genes show over 90% homology. NGF stimulates growth and differentiation of sympathetic and sensory neurons.
Mutations in the NGF gene are associated with hereditary sensory and autonomic neuropathy type 5 (HSAN5), and dysregulation of NGF expression is linked to allergic rhinitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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