Thermo Fisher Scientific Ferritin, Heavy Chain (FTH) Monoclonal Antibody (FTH, 2081)
Ferritin Heavy Chain(FTH) 단백질을 인식하는 mouse monoclonal antibody로, WB 및 IHC(P) 분석에 적합. Recombinant FTH1(aa58-180)을 면역원으로 제작. Protein A/G 정제, 200 µg/mL 농도, PBS buffer에 0.05% BSA 및 sodium azide 포함. 연구용 한정 제품.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.1–0.2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2c, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FTH, 2081 |
| Immunogen | Recombinant human FTH1 protein fragment (around aa 58–180) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
FTH1 is the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It consists of 24 subunits of heavy and light ferritin chains.
Variation in ferritin subunit composition may affect iron uptake and release rates in different tissues.
Ferritin’s main function is storing iron in a soluble and nontoxic state.
Defects in ferritin proteins are associated with several neurodegenerative diseases.
This gene has multiple pseudogenes and several alternatively spliced transcript variants, though their biological validity remains unclear.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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