Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), Brilliant Ultra Violet 563, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496 / 615 / 661 / 737
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue / Red / Yellow series
• PE, PE-Cyanine5 / 5.5 / 7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 780
• Custom conjugation available

Product Specific Information

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. CD3e, together with CD3γ and CD3δ, is essential for proper assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Binding of 145-2C11 to TCR initiates intracellular signaling leading to activation, proliferation, or apoptosis depending on conditions.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume). Optimal titration recommended.

Fluorophore Information:
Brilliant Ultra Violet™ 563 (BUV563) emits at 564 nm and is designed for use with UV (355 nm) lasers. Confirm instrument compatibility before use.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be fixed using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal signal loss. Clone-specific performance should be validated empirically.

Excitation: 350 nm
Emission: 564 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

The CD3 subunit complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. CD3 subunits (γ, δ, ε, ζ) are immunoglobulin superfamily members encoded on chromosome 11. They associate with cytoplasmic signaling molecules via tyrosine-based motifs. CD3 is expressed on most T lymphocytes and thymocytes, but not on B or NK cells. CD3 defects are linked to immunodeficiency disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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