Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), Brilliant Ultra Violet 496, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤ 0.5 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 661, 737
• Brilliant Violet 421
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 510–800 Series
• NovaFluor UV 765, Violet 690–800, Yellow 810
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 600–780
• Request custom conjugation

Product Specific Information

Description:
The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and by most peripheral T cells. Crosslinking of the TCR complex with H57-597 can induce T cell activation, proliferation, or apoptosis depending on assay conditions. It is used as a phenotypic marker for TCR beta-expressing T cells.

Applications Reported:
Flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes at ≤ 0.5 µg/test (100 µL final volume). Optimize antibody titration for best performance.

Fluorophore Information:
Brilliant Ultra Violet™ 496 (BUV496) emits at 496 nm and is suitable for cytometers equipped with a 355 nm UV laser. Confirm instrument compatibility prior to use.

Staining Recommendations:
When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)

Excitation/Emission:

• Excitation: 350 nm
• Emission: 496 nm
• Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

T cell receptors (TCRs) enable T cells to distinguish foreign from self-peptides presented by MHC class II molecules, crucial for adaptive immunity. Abnormal TCR recognition of self-peptides is linked to autoimmune diseases and tumor-associated mutant peptides. Superantigen engagement of TCRs can cause toxic shock syndrome. Autoantibodies to TCR V beta segments have been identified in rheumatoid arthritis and systemic lupus erythematosus, potentially modulating inflammatory responses.

Most human T cells express TCR alpha-beta with CD4 or CD8 (single positive), while a small subset lacks both (double negative). Increased alpha-beta DN T cells are found in autoimmune and immunodeficiency disorders. Gamma-delta T cells, primarily epithelial, exhibit limited TCR diversity and distinct antigen recognition, with subsets showing antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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