Thermo Fisher Scientific SIGLEC H Monoclonal Antibody (eBio440c), eFluor 660, eBioscience

Thermo Fisher Scientific SIGLEC H Monoclonal Antibody (eBio440c), eFluor 660, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test
Publications: View 3 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• Brilliant UV 615
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on plasmacytoid dendritic cells (pDC) or type I IFN-producing cells (IPC) in the naive mouse.
Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and respond rapidly to viral infection.

Siglec-H is a transmembrane protein of the Ig superfamily that binds sialic acid but lacks the cytoplasmic ITIM domain. It associates with DAP12 for signal transduction.
The eBio440c antibody inhibits pDC function (reduces IFNα secretion in response to CpG).
Some mouse strains, such as SJL, have higher pDC percentages compared to C57BL/6.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤0.25 µg/test (100 µL final volume).
Cell number should be optimized empirically (10⁵–10⁸ cells/test).
Careful titration is recommended for best performance.

Fluorophore Characteristics:
eFluor™ 660 replaces Alexa Fluor™ 647.
Emission: 659 nm | Excitation: 633 nm | Laser: Red.
Filtered post-manufacturing (0.2 µm).

Target Information

Siglec-H is expressed on plasmacytoid dendritic cells (pDC) or type I IFN-producing cells (IPC) in naive mice.
It binds sialic acid and associates with DAP12 for signaling.
eBio440c antibody inhibits pDC function, reducing IFNα secretion in response to CpG.
Mouse strain differences in pDC levels have been observed (e.g., SJL vs. C57BL/6).

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

제품 이미지