Thermo Fisher Scientific p21WAF1 (Tumor Suppressor Protein) Monoclonal Antibody (SPM306)
p21WAF1 종양 억제 단백질을 인식하는 단클론 항체로, 다양한 면역분석(WB, IHC, ICC, Flow)에 사용 가능. 인간 시료에 반응하며 Mouse, Rat에는 반응하지 않음. Protein A/G로 정제된 액상 형태로 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 2–4 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM306 |
| Immunogen | Human recombinant p21 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Mouse or Rat.
Target Information
Protein p21 is a tumor suppressor protein that inhibits Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53.
p21 plays a key role in the cellular response to genotoxic stress and serves as a major transcriptional target of p53.
In the nucleus, p21 binds to and inhibits Cdk1 and Cdk2, blocking the transition from G1 to S phase or from G2 to mitosis after DNA damage, allowing DNA repair.
In the cytoplasm, p21 exhibits anti-apoptotic activity by inhibiting caspase 3 and apoptotic kinases ASK1 and JNK.
p21 has dual roles in carcinogenesis, functioning as both a tumor suppressor and an oncogene depending on the context.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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