Thermo Fisher Scientific NSE Polyclonal Antibody
Thermo Fisher Scientific의 NSE Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하는 토끼 유래 다클론 항체입니다. Western blot과 ELISA에 적합하며, 신경 특이적 에놀라제(NSE)를 검출합니다. 세포막 및 세포질 내 단백질 분석에 유용합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–127 of human ENO2 (NP_0019661) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.85 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914428 |
Product Specific Information
Positive test controls include: SH-SY5Y, OVCAR3, 22Rv1, SW620, A-549, Jurkat, Mouse brain, Mouse spinal cord, Mouse eye.
The target is usually found in the following locations: Cell membrane, Cytoplasm.
Immunogen sequence:
MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERE
Target Information
Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and the reverse reaction in gluconeogenesis.
NSE has high stability in biological fluids and diffuses easily into extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.
It is one of three mammalian enolases (ENO1, ENO2, ENO3), also known as enolase alpha, beta, and gamma.
- Alpha-subunit: expressed in most tissues
- Beta-subunit: expressed only in muscle
- Gamma-subunit: expressed primarily in neurons and neuroendocrine cells
Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms.
Antibodies to NSE are useful for identifying neuronal cell bodies, developing neuronal lineage, and neuroendocrine cells.
NSE release into CSF and blood serves as a biomarker of neuronal injury and is clinically used as a marker of neuronal damage in disorders such as stroke, hypoxic brain injury, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis.
Elevated NSE levels are also observed in neuroblastoma and small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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