Thermo Fisher Scientific CD63 Monoclonal Antibody (MEM-259), FITC
CD63 단백질을 인식하는 Mouse IgG1 단클론 항체로 FITC 형광 표지. 인간 세포에 반응하며 Flow Cytometry 및 Immunocytochemistry에 적합. 활성화 의존적 CD63 표면 발현 분석에 유용. 연구용으로만 사용.
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Thermo Fisher Scientific CD63 Monoclonal Antibody (MEM-259), FITC
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | Assay-dependent | View 1 publication |
| Flow Cytometry (Flow) | 20 µL × 100 µL whole blood or 10⁶ cells | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-259 |
| Immunogen | T cell line HPB-ALL |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1073280 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- APC
- PE
- PerCP
- Custom conjugation available upon request
Product Specific Information
This antibody reacts with an extracellular/luminal epitope of CD63 (LAMP-3), a 40–60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages, and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.
Target Information
CD63 (LAMP-3, lysosome-associated membrane protein-3) is a tetraspanin family glycoprotein located in late endosomes, lysosomes, and secretory vesicles. Upon cell activation, CD63 translocates to the plasma membrane and serves as a basophil activation marker. In mast cells, CD63 exposition does not require activation. CD63 interacts with integrins, modulates phagocytosis and cell migration, and participates in H/K-ATPase trafficking and ROMK1 channel regulation. It also functions as a T-cell costimulatory molecule and is associated with melanoma and Hermansky-Pudlak Syndrome. Expression levels can be used for carcinoma prognosis evaluation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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