Thermo Fisher Scientific HSP27 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

PA1-017 detects heat shock protein 27 (HSP27) from rat, mouse, monkey, and human tissues.
This antibody has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunohistochemistry.
In Western blot, it detects a ~27 kDa protein corresponding to HSP27 from HeLa cells.
Immunocytochemical staining shows diffuse cytoplasmic staining before heat shock and both diffuse cytoplasmic and punctate staining patterns after heat shock.

The immunogen is a synthetic peptide corresponding to residues L(10) L R G P S W D P F R C(21) of human HSP27, which is 81% conserved in mice, rats, and hamsters.
Phosphorylated peptide (Cat. # PEP-187) and non-phosphorylated peptide (Cat. # PEP-240) are available for neutralization and control experiments.

Target Information

Cells respond to environmental stress by increasing expression of heat shock or stress proteins.
HSPB1 (heat shock protein beta-1 or HSP27) acts as a molecular chaperone, maintaining denatured proteins in a folding-competent state.
It contributes to stress resistance and actin organization, regulating phosphorylation and axonal transport of neurofilament proteins.
Mutations in the HSPB1 gene can lead to Charcot-Marie-Tooth disease 2F and distal hereditary motor neuronopathy type 2B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.