Thermo Fisher Scientific GAPDH Loading Control Monoclonal Antibody (GA1R)
GAPDH 단백질 검출용 모노클로날 항체로, 다양한 종에서 반응하며 Western blot, IHC, ICC 등 다중 응용에 사용 가능. 단백질 로딩 컨트롤용으로 적합하며, 고순도 Protein A 정제 및 안정적 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | 407 |
| Immunohistochemistry (IHC) | – | 1 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:500 | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | – | 1 |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 | 64 |
| Flow Cytometry (Flow) | 2 µg/test | 1 |
| ELISA | Assay-dependent | – |
| Immunoprecipitation (IP) | – | 2 |
| Functional Assay | – | 1 |
| in situ PLA (PLA) | – | 2 |
| In vitro Assay (IV) | – | 1 |
| Miscellaneous PubMed (Misc) | – | 15 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria, Dog, Chicken, Hamster, Human, Insect, Mouse, Rabbit, Rat, Yeast |
| Published Species | Bacteria, Chicken, Dog, Fruit fly, Horse, Human, Mouse, Non-human primate, Pig, Protozoa, Rabbit, Rat, Rhesus monkey, Rodent, Yeast, Zebrafish |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GA1R |
| Immunogen | Recombinant GAPDH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10977387 |
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 647
- Biotin
- DyLight 488
- DyLight 550
- DyLight 650
- DyLight 680
- DyLight 800 4X PEG
- HRP
- Custom conjugation available upon request
Product Specific Information
This antibody detects GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, hamster, and chicken samples.
MA5-15738 has been successfully used in Western blot, ICC, IF, IHC (P), FACS, and ELISA.
MA5-15738 may be stored at 4ºC short term. Add 0.05% sodium azide if desired. For long term storage, store at -20ºC, avoiding freeze/thaw cycles.
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis, existing as a tetramer of identical 37-kDa subunits. It catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond its glycolytic role, GAPDH participates in DNA replication, DNA repair, RNA export, membrane fusion, and microtubule bundling. It is implicated in apoptosis, neurodegenerative diseases, and cancer-related processes.
Due to its ubiquitous expression and consistent levels across tissues, GAPDH is widely used as a protein loading control in Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
배송 정보
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