Thermo Fisher Scientific GAPDH Loading Control Monoclonal Antibody (GA1R)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 407 publications 407 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:20-1:200

View 64 publications 64 publications

Flow Cytometry (Flow)

2 µg/test

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Functional Assay (Functional)

-

View 1 publication 1 publication

in situ PLA (PLA)

-

View 2 publications 2 publications

In vitro Assay (IV)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 15 publications 15 publications

Product Specifications

Species Reactivity

Bacteria, Dog, Chicken, Hamster, Human, Insect, Mouse, Rabbit, Rat, Yeast

Published species

Bacteria, Chicken, Dog, Fruit fly, Horse, Human, Mouse, Non-human primate, Pig, Protozoa, Rabbit, Rat, Rhesus monkey, Rodent, Yeast, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GA1R

Immunogen

Recombinant GAPDH

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 555
• Alexa Fluor 647
• Biotin
• DyLight 488
• DyLight 550
• DyLight 650
• DyLight 680
• DyLight 800 4X PEG
• HRP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10977387

Product Specific Information

This antibody detects GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, hamster, and chicken samples.

MA5-15738 has been successfully used in Western blot, ICC, IF, IHC (P), FACS and ELISA.

MA5-15738 may be stored at 4ºC short term. Add 0.05% sodium azide if desired. For long term storage, store at -20ºC, avoiding freeze/thaw cycles.

Target Information

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimers disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntingtons disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.