Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Super Bright 702, eBioscience
마우스 CD4 분자를 인식하는 GK1.5 클론 단일클론 항체로, Super Bright 702 형광체로 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, 405 nm 레이저로 여기되고 702 nm에서 방출됩니다. 높은 특이성과 안정적인 형광 신호를 제공하며, 연구용으로 적합합니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Super Bright 702, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 6 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 90 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 702, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GK1.5 |
| Conjugate | Super Bright™ 702 |
| Excitation / Emission Max | 413 / 702 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2722962 |
Available Formats
Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395, Brilliant UV 563, Brilliant UV 661, Brilliant UV 737, Brilliant Violet 480, Brilliant Violet 605, eFluor 450, eFluor 506, eFluor 660, FITC, Functional Grade, NovaFluor Blue/Red/Violet/Yellow series, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610, PerCP-eFluor 710, Super Bright 436, Super Bright 600, Super Bright 645, Super Bright 780, and custom conjugation available.
Product Specific Information
The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, mature T cell subsets, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and is essential for T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on mouse splenocytes, ≤0.25 µg/test (100 µL final volume)
- Excitation/Emission: 405 nm / 702 nm (Violet laser recommended, 710/50 bandpass filter)
- Light Sensitivity: Protect from light
- Fixation: Samples can be stored in IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days at 4°C in the dark
- Recommended Buffer: Super Bright Complete Staining Buffer (Product # SB-4401) to minimize polymer interactions
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD4 antigen is involved in recognition of MHC class II molecules and acts as a co-receptor for HIV. It is primarily expressed on T helper cells but also found on monocytes, macrophages, and dendritic cells. Tissue-level expression includes thymus, lymph nodes, tonsils, spleen, and specific regions of the brain and gut. CD4 functions in T-cell activation and may mediate neuronal damage in immune-related diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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