Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Super Bright 702, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395, Brilliant UV 563, Brilliant UV 661, Brilliant UV 737, Brilliant Violet 480, Brilliant Violet 605, eFluor 450, eFluor 506, eFluor 660, FITC, Functional Grade, NovaFluor Blue/Red/Violet/Yellow series, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610, PerCP-eFluor 710, Super Bright 436, Super Bright 600, Super Bright 645, Super Bright 780, and custom conjugation available.

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, mature T cell subsets, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and is essential for T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes, ≤0.25 µg/test (100 µL final volume)
• Excitation/Emission: 405 nm / 702 nm (Violet laser recommended, 710/50 bandpass filter)
• Light Sensitivity: Protect from light
• Fixation: Samples can be stored in IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days at 4°C in the dark
• Recommended Buffer: Super Bright Complete Staining Buffer (Product # SB-4401) to minimize polymer interactions

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD4 antigen is involved in recognition of MHC class II molecules and acts as a co-receptor for HIV. It is primarily expressed on T helper cells but also found on monocytes, macrophages, and dendritic cells. Tissue-level expression includes thymus, lymph nodes, tonsils, spleen, and specific regions of the brain and gut. CD4 functions in T-cell activation and may mediate neuronal damage in immune-related diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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