Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Super Bright 702, eBioscience
마우스 CD4 항원 인식용 RM4-5 클론 단클론 항체로, Super Bright 702 형광체 결합. Flow cytometry에 최적화되어 있으며 0.125 µg/test로 사용 가능. 405 nm 레이저에서 여기되어 702 nm로 방출. 광민감성이 있으므로 암조건에서 보관 권장.
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Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Super Bright 702, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 8 publications |
| Immunohistochemistry (PFA fixed) | - | View 1 publication |
| Immunohistochemistry (Frozen) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 73 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 702, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RM4-5 |
| Conjugate | Super Bright™ 702 |
| Excitation / Emission Max | 413 / 702 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2662399 |
Available Formats
- Unconjugated
- Alexa Fluor 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805
- Brilliant Violet 421–786
- eFluor 450–615
- FITC
- NovaFluor Blue & Yellow Series
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 780
- Request custom conjugation
Product Specific Information
The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by thymocytes, mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and plays a key role in T cell development and function. RM4-5 binding is blocked by GK1.5.
- Applications Tested: Flow cytometric analysis of mouse thymocytes and splenocytes. Recommended ≤0.125 µg/test (100 µL total volume).
- Super Bright 702 Dye: Excited by 405 nm violet laser, emits at 702 nm. Use 710/50 bandpass filter.
- Staining Panels: When using multiple Super Bright dyes, use Super Bright Staining Buffer (SB-4401-42) to minimize non-specific interactions.
- Light Sensitivity: Protect vial and samples from light.
- Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333).
Excitation: 405 nm
Emission: 702 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD4 antigen is involved in MHC class II recognition and acts as a co-receptor for HIV. Expressed mainly in T helper cells, and also in monocytes, macrophages, and dendritic cells. Tissue-level expression includes thymus, lymph nodes, tonsils, spleen, and specific regions of brain and gut. CD4 aids early T-cell activation via association with Lck kinase and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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