Thermo Fisher Scientific PHLPP2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:1,000

Product Specifications

Product Specific Information

• Recommended positive controls: Mouse brain
• Predicted reactivity: Mouse (96%), Rat (97%), Bovine (95%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on Ser-473 of AKT1, Ser-660 of PRKCB isoform beta-II, and Ser-657 of PRKCA.
Akt regulates the balance between cell survival and apoptosis through a cascade that alters transcription factors controlling pro- and antiapoptotic genes.
Dephosphorylation of Ser-473 of Akt triggers apoptosis and decreases cell proliferation. Also controls phosphorylation of AKT3. Dephosphorylates STK4 on Thr-387 leading to activation and apoptosis (PubMed:20513427).
Dephosphorylates RPS6KB1 and regulates cap-dependent translation (PubMed:21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (PubMed:24530606). [UniProt]

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 파일: PA5-31319_PHLPP2_Q6ZVD8-1_Rabbit.svg, PA5-31319_PHLPP2_Q6ZVD8-1_Rabbit_PDP.jpeg)